5FCK
COMPLEMENT FACTOR D IN COMPLEX WITH COMPOUND 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-24 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.266, 48.742, 54.730 |
| Unit cell angles | 90.00, 108.82, 90.00 |
Refinement procedure
| Resolution | 22.870 - 1.860 |
| R-factor | 0.18935 |
| Rwork | 0.187 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fbe |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.914 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.870 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.059 | 0.194 |
| Number of reflections | 14490 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 89.8 | 49.8 |
| Redundancy | 2.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1 uL FD solution (18 mg/mL FD, 10 mM Tris pH 7.0, 100 mM NaCl) was mixed with 1 uL reservoir solution (25% PEG3350, 100 mM TRIS pH 8.0) and equilibrated against 1 mL reservoir solution. |
