5FBK
Crystal structure of the extracellular domain of human calcium sensing receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.872, 82.916, 94.256 |
| Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
| Resolution | 37.770 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.22270 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.379 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.072 | 0.029 | 0.913 |
| Rmeas | 0.076 | 0.034 | |
| Rpim | 0.043 | 0.017 | 0.541 |
| Total number of observations | 284906 | ||
| Number of reflections | 74547 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 3.8 | 3.7 | 3.8 |
| CC(1/2) | 0.998 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 10% PEG 8000, 200 mM MgCl2, 10 mM CaCl2 and 100 mM Tris-HCl |
