5F6Z
Sandercyanin Fluorescent Protein purified from Sander vitreus
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-08-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 93.394, 93.394, 246.426 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 19.835 - 2.248 |
| R因子 | 0.2398 |
| Rwork | 0.237 |
| R-free | 0.31160 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.288 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | d*TREK |
| Refinement software | PHENIX (1.8.1_1168) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 19.840 | 2.329 |
| 分解能 [Å] (高) | 2.248 | 2.248 |
| 独立反射数 | 52052 | |
| <I/σ(I)> | 5.86 | |
| 完全性 [%] | 98.6 | |
| 冗長性 | 3.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 0.1M sodium acetate, 3M magnesium sulphate hydrate, 20% PEG 8000 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 0.1M sodium acetate, 3M magnesium sulphate hydrate, 20% PEG 8000 |
