5F5X
Crystal structure of S116A Ba3275 with AMP bound
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 55.116, 117.728, 54.852 |
| 格子定数 [度] | 90.00, 95.27, 90.00 |
精密化法
| 残基 | 31.922 - 2.006 |
| R因子 | 0.2149 |
| Rwork | 0.213 |
| R-free | 0.24660 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.731 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1888) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 32.000 |
| 分解能 [Å] (高) | 2.000 |
| Rmerge_l_obs | 0.087 |
| 独立反射数 | 45151 |
| <I/σ(I)> | 11.6 |
| 完全性 [%] | 97.6 |
| 冗長性 | 2.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | Malate pH 5.0 0.15M, Peg 1000 %(w/v), Sodium fluoride 0.004 M |
