5EW5
Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.894, 116.050, 150.365 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 29.600 - 3.200 |
| R-factor | 0.21544 |
| Rwork | 0.212 |
| R-free | 0.27062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JCH and 2GZG |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 53941 | |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 99.1 | 95.7 |
| Redundancy | 3.5 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 1.3M sodium malonate, PEG3350 |
