5E8K

Crystal structure of polyprenyl pyrophosphate synthase 2 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2013-10-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.987
Spacegroup name	I 4
Unit cell lengths	150.460, 150.460, 74.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.028
R-factor	0.2304
Rwork	0.229
R-free	0.25730
RMSD bond length	0.012
RMSD bond angle	1.965
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	16306
<I/σ(I)>	10.9
Completeness [%]	99.7
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	293	5% isopropanol, 8% PEG 4000, pH5.9