5E3M
Crystal structure of Fis bound to 27bp DNA F35 (AAATTAGTTTGAATCTCGAGCTAATTT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.880, 43.220, 97.160 |
| Unit cell angles | 90.00, 111.52, 90.00 |
Refinement procedure
| Resolution | 40.412 - 2.886 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.776 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.14) |
| Phasing software | PHASER (1.3.2) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.412 | 40.410 | 3.040 |
| High resolution limit [Å] | 2.880 | 9.120 | 2.880 |
| Rmerge | 0.082 | 0.050 | 0.916 |
| Rpim | 0.034 | 0.020 | 0.370 |
| Total number of observations | 89910 | 2871 | 14309 |
| Number of reflections | 13581 | ||
| <I/σ(I)> | 13.7 | 27.1 | 2.4 |
| Completeness [%] | 94.4 | 96.1 | 99 |
| Redundancy | 6.6 | 5.9 | 7.1 |
| CC(1/2) | 0.998 | 0.998 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400 |
