5E3A
Structure of human DPP3 in complex with opioid peptide leu-enkephalin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972390 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 119.558, 105.757, 64.992 |
| 格子定数 [度] | 90.00, 93.43, 90.00 |
精密化法
| 残基 | 39.576 - 2.050 |
| R因子 | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t6b |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.616 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 39.580 | 2.121 |
| 分解能 [Å] (高) | 2.050 | 2.050 |
| Rmerge_l_obs | 0.089 | 0.620 |
| 独立反射数 | 48267 | |
| <I/σ(I)> | 10.73 | 1.85 |
| 完全性 [%] | 95.1 | 95.45 |
| 冗長性 | 3 | 3.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.056M SODIUM PHOSPHATE MONOBASIC MONOHYDRATE, 1.344M POTASSIUM PHOSPHATE DIBASIC |
