5DQZ
Crystal Structure of Cas-DNA-PAM complex
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 73.771, 193.380, 193.230 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 29.534 - 2.700 |
R因子 | 0.1987 |
Rwork | 0.197 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p6i |
結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
結合角の平均二乗偏差(RMSD) [度] | 0.979 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 50.000 | 50.000 | 2.800 |
分解能 [Å] (高) | 2.700 | 5.810 | 2.700 |
Rmerge_l_obs | 0.113 | 0.044 | |
Total number of observations | 590102 | ||
独立反射数 | 76448 | ||
<I/σ(I)> | 7.8 | ||
完全性 [%] | 99.5 | 96.2 | 99.6 |
冗長性 | 7.7 | 7.1 | 7.8 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 50mM Na cacodylate,0.9mM spermine,18mM Magnesium chloride, 1.8mM Cobalt chloride, 9% isopropanol |