5DQT
Crystal Structure of Cas-DNA-22 complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 71.434, 194.955, 195.202 |
| 格子定数 [度] | 90.00, 90.22, 90.00 |
精密化法
| 残基 | 48.800 - 3.100 |
| R因子 | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 3.150 |
| 分解能 [Å] (高) | 3.100 | 8.400 | 3.100 |
| Rmerge_l_obs | 0.082 | 0.044 | 0.670 |
| Rmeas | 0.097 | 0.051 | 0.813 |
| Rpim | 0.051 | 0.026 | 0.451 |
| Total number of observations | 274007 | ||
| 独立反射数 | 91533 | ||
| <I/σ(I)> | 11.2 | ||
| 完全性 [%] | 93.3 | 97 | 96.7 |
| 冗長性 | 3 | 3.4 | 2.8 |
| CC(1/2) | 0.995 | 0.355 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 5% PEG8000,50mM Mes,200mM Potassium chloride,10mM Magnesium chloride |
