5DLJ
Crystal Structure of Cas-DNA-N1 complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 69.894, 193.652, 193.920 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 39.835 - 2.600 |
| R因子 | 0.1808 |
| Rwork | 0.179 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.050 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.690 |
| 分解能 [Å] (高) | 2.600 | 5.600 | 2.600 |
| Rmerge_l_obs | 0.088 | 0.040 | 0.807 |
| Total number of observations | 499478 | ||
| 独立反射数 | 82019 | ||
| <I/σ(I)> | 8.9 | ||
| 完全性 [%] | 99.9 | 99.7 | 99.8 |
| 冗長性 | 6.1 | 6.3 | 5.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 10% PEG400,50mM Na cacodylate,3mM spermine,15mM Magnesium chloride |
