5DGY

Crystal structure of rhodopsin bound to visual arrestin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-20
Detector	PSI PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 21
Unit cell lengths	107.484, 107.258, 460.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.978 - 7.700
R-factor	0.2877
Rwork	0.285
R-free	0.33510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zwj
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	8.100
High resolution limit [Å]	7.700	7.700
Rmerge	0.314	1.188
Number of reflections	6795
<I/σ(I)>	3.79	1.08
Completeness [%]	97.5	0.908
Redundancy	4.68	4.12
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	PEG400