5CB2
the structure of candida albicans Sey1p in complex with GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 76.566, 120.926, 190.455 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.26156 |
Rwork | 0.260 |
R-free | 0.29806 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.639 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 19897 |
<I/σ(I)> | 27.2 |
Completeness [%] | 99.5 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 120 Mm DL-Malic acid pH 7.0, 16% w/v PEG 3350, 20 mM Bicine pH 9.0, 400 mM Magnesium chloride hexahydrate, 6% D-(+)-Trehalose dihydrate |