5BMV
CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-Vinblastine COMPLEX
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 105.470, 157.420, 183.180 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.500 |
| R因子 | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tv8 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.727 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 50.000 |
| 分解能 [Å] (高) | 2.500 |
| 独立反射数 | 106106 |
| <I/σ(I)> | 19 |
| 完全性 [%] | 100.0 |
| 冗長性 | 6.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 293 | 6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2 |
