5B4Y
Crystal structure of the LA12 fragment of ApoER2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.162, 41.780, 55.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.280 - 1.900 |
R-factor | 0.21197 |
Rwork | 0.210 |
R-free | 0.24667 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a7q |
RMSD bond length | 0.012 |
RMSD bond angle | 1.674 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.800 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.086 | |
Number of reflections | 6043 | |
<I/σ(I)> | 9.5 | |
Completeness [%] | 99.9 | |
Redundancy | 5.2 | |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20%(wt./vol.) PEG monomethylether 2000, 100 mM Tris-Cl (pH 7.0) |