5B4X
Crystal structure of the ApoER2 ectodomain in complex with the Reelin R56 fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97904 |
Spacegroup name | P 65 |
Unit cell lengths | 205.951, 205.951, 169.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.520 - 3.200 |
R-factor | 0.20277 |
Rwork | 0.200 |
R-free | 0.25481 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.520 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.139 | |
Number of reflections | 67441 | |
<I/σ(I)> | 16.4 | |
Completeness [%] | 100.0 | |
Redundancy | 14.8 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 11-12.5%(wt./vol.) PEG 3350 200-250mM sodium thiocyanate, 20mM HEPES-Na (pH 7.5) |