5AYK
Crystal structure of ERdj5 form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.10 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.990, 52.970, 92.900 |
Unit cell angles | 90.00, 113.54, 90.00 |
Refinement procedure
Resolution | 19.951 - 2.250 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.24320 |
Structure solution method | MIR |
Starting model (for MR) | 3apo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.837 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.140 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 39380 | |
<I/σ(I)> | 11 | 1.3 |
Completeness [%] | 99.4 | 94.16 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG8000, cystin |