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5AX0

Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.52 angstrom

Replaces:  3WT9
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL32XU
Synchrotron siteSPring-8
BeamlineBL32XU
Temperature [K]100
Detector technologyCCD
Collection date2010-07-27
DetectorRAYONIX MX225HE
Wavelength(s)1.0
Spacegroup nameC 1 2 1
Unit cell lengths75.990, 101.630, 43.760
Unit cell angles90.00, 119.17, 90.00
Refinement procedure
Resolution38.210 - 1.521
R-factor0.1767
Rwork0.176
R-free0.19630
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1c3w
RMSD bond length0.006
RMSD bond angle0.919
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwarePHENIX (1.9_1690)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]38.2101.580
High resolution limit [Å]1.5201.520
Number of reflections44164
<I/σ(I)>12.191.77
Completeness [%]99.497.93
Redundancy3.83.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1LIPIDIC CUBIC PHASE729318% PEG400, 0.1M Hepes/NaOH, 0.2M NaCl, 10mM MgCl2, 2.5% MPD

217705

건을2024-03-27부터공개중

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