5AWZ
Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.57 angstrom
「3WT8」から置き換えられましたExperimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 75.990, 101.630, 43.760 |
| 格子定数 [度] | 90.00, 119.17, 90.00 |
精密化法
| 残基 | 30.540 - 1.570 |
| R因子 | 0.1776 |
| Rwork | 0.177 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.122 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1690) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.540 | 1.630 |
| 分解能 [Å] (高) | 1.570 | 1.570 |
| 独立反射数 | 39721 | |
| <I/σ(I)> | 9.87 | 1.82 |
| 完全性 [%] | 98.3 | 83.67 |
| 冗長性 | 3.8 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 18% PEG400, 0.1M sodium cacodylate, 0.2M NaCl, 10mM MgCl2, 2.5% MPD |
