5A16
Crystal structure of Fab4201 raised against Human Erythrocyte Anion Exchanger 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 82.190, 82.190, 270.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.514 - 2.500 |
| R-factor | 0.2149 |
| Rwork | 0.213 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r8s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.933 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.480 |
| Number of reflections | 61639 | |
| <I/σ(I)> | 9 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% (W/V) PEG20000, 0.1% NA ACETATE (PH5.3) |
