5T9E

Seleno-methionine Prephenate Dehydrogenase from Soybean

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	46.508, 55.127, 68.592
Unit cell angles	107.34, 98.89, 103.55

Refinement procedure

Resolution	34.060 - 2.030
R-factor	0.19
Rwork	0.188
R-free	0.22900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.172
Phasing software	MLPHARE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.030
High resolution limit [Å]	2.030	2.000
Rmerge	0.068	0.328
Number of reflections	60106
<I/σ(I)>	7.6	1.6
Completeness [%]	96.0	88.7
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% (w/v) PEG-3350, 100 mM sodium citrate, pH 4.0, and 200 mM sodium citrate tribasic