5J5J

Crystal structure of a chimera of human Desmocollin-2 EC1 and human Desmoglein-2 EC2-EC5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	63.494, 87.489, 218.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.295 - 3.290
R-factor	0.2489
Rwork	0.247
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iry 5erd
RMSD bond length	0.027
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1810)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.295	3.550
High resolution limit [Å]	3.290	3.300
Rmerge		1.577
Number of reflections	19019
<I/σ(I)>	10.1	1.1
Completeness [%]	99.4	98.5
Redundancy	5.8	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	33% (w/v) Ethylene glycol-PEG 8000, 0.1M Tris-bicine pH 8.5, 10%(v/v) monosaccharides mix (Molecular Dimensions), 0.01M calcium chloride