4ZTS
Human Aurora A catalytic domain bound to FK1142
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.380, 82.380, 188.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.170 - 2.900 |
| R-factor | 0.21296 |
| Rwork | 0.211 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.034 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.630 |
| Number of reflections | 8932 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 4.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 5% MPD, 0.1 M Hepes pH 7.5, 10% PEG 10K |
