4ZIX
Structure of HEWL using Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2014-02-27 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.56 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.100, 79.100, 38.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.966 - 1.890 |
R-factor | 0.1665 |
Rwork | 0.165 |
R-free | 0.19070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.713 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.970 |
High resolution limit [Å] | 1.890 |
Number of reflections | 10266 |
<I/σ(I)> | 9.5 |
Completeness [%] | 100.0 |
Redundancy | 26558 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 3.5 | 293 | Lysozyme powder from chicken egg white was dissolved in 0.02 M Sodium acetate pH 4.6 buffer to a final concentration of 50 mg/mL. Crystallization was initiated by injecting 20 uL of lysozyme solution (50 mg/mL) into 1 mL of precipitant solution (20 %(w/v) NaCl, 6 %(v/v) PEG 6000, 1 M Na acetate pH 3.0) at room temperature. |