4ZIC
Crystal Structure of Aspartate Semialdehyde Dehydrogenase with NADP from Trichophyton rubrum
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97876 |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 157.427, 157.427, 187.987 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 46.133 - 2.553 |
| R因子 | 0.229 |
| Rwork | 0.227 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zhs |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.056 |
| Refinement software | PHENIX (1.7.3_928) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.710 |
| 分解能 [Å] (高) | 2.550 | 2.550 |
| Rmerge_l_obs | 0.056 | 0.336 |
| 独立反射数 | 168846 | |
| <I/σ(I)> | 19.84 | 4.37 |
| 完全性 [%] | 99.7 | 99.4 |
| 冗長性 | 3.84 | 3.83 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.15M Ammonium Sulfate, 0.1M Sodium Citrate, 15% PEG 4000 |
