4ZH6
Crystal Structure of the Domain-Swapped Dimer Y60L mutant of Human Cellular Retinol Binding Protein II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 36.776, 60.424, 63.603 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.837 - 1.550 |
R-factor | 0.1902 |
Rwork | 0.187 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.006 |
RMSD bond angle | 0.959 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.588 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.052 | 0.695 |
Number of reflections | 21293 | |
<I/σ(I)> | 82.03 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 25% PEG 4000 (40%), 0.1M Sodium Acetate pH 4.5, Ammonium Acetate 0.1M |