4ZDS

Crystal Structure of core DNA binding domain of Arabidopsis Thaliana Transcription Factor Ethylene-Insensitive 3

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1
格子定数 [Å]	35.022, 45.174, 47.571
格子定数 [度]	89.46, 77.07, 71.38

精密化法

残基	46.260 - 1.780
R因子	0.17652
Rwork	0.174
R-free	0.21806
結合長の平均二乗偏差(RMSD) [Å]	0.019
結合角の平均二乗偏差(RMSD) [度]	2.126
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Quality characteristics

	Overall
分解能 [Å] (低)	46.260
分解能 [Å] (高)	1.780
独立反射数	23591
<I/σ(I)>	22.81
完全性 [%]	96.6
冗長性	3.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1M HEPES, 25% w/v Polyethylene glycol 3350