4ZCB
Human CRBPII mutant - Y60W dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-10-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.1271 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 62.620, 109.774, 36.322 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.590 - 1.700 |
R-factor | 0.18487 |
Rwork | 0.182 |
R-free | 0.23884 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qyn |
RMSD bond length | 0.024 |
RMSD bond angle | 2.112 |
Data reduction software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 26934 | |
<I/σ(I)> | 66.13 | 7.71 |
Completeness [%] | 98.6 | |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 30% PEG 4000, 0.1M CH3COONa.3H2O, pH 4.6, 0.1M CH3COONH4 |