4Z5H

HipB(S29A)-O2 20mer complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-02-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 3 2
格子定数 [Å]	127.714, 127.714, 127.714
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	45.154 - 2.100
R因子	0.1879
Rwork	0.187
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4Z52
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.259
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	50.000	50.000	2.140
分解能 [Å] (高)	2.100	5.700	2.100
Rmerge_l_obs	0.053	0.023	0.914
Total number of observations	479223
独立反射数	21371
<I/σ(I)>	15
完全性 [%]	100.0	100	100
冗長性	22.4	20.7	21.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5	298	1 M sodium citrate