4YZF
Crystal structure of the anion exchanger domain of human erythrocyte Band 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-03-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9173 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.825, 171.957, 271.700 |
Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
Resolution | 37.718 - 3.500 |
R-factor | 0.2752 |
Rwork | 0.274 |
R-free | 0.29010 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.369 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: dev_1951)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.780 | |
Number of reflections | 84446 | |
<I/σ(I)> | 18 | 1.6 |
Completeness [%] | 97.3 | 96.5 |
Redundancy | 5.6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 100 mM Tris-HCl (pH 8.0), 22-26 %(v/v) PEG300, 250 mM CH3COOK |