4YSL
Crystal structure of SdoA from Pseudomonas putida in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-01-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 |
Unit cell lengths | 79.625, 79.625, 93.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.459 - 1.462 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.20370 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.546 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.460 |
Number of reflections | 100527 |
<I/σ(I)> | 30.6 |
Completeness [%] | 98.2 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 200 mM Ammonium acetate, 100 mM HEPES (pH 7.5), and 25% (w/v) PEG 3350 |