4YAL

Reduced CYPOR with 2'-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	101.907, 115.418, 118.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.610 - 1.880
R-factor	0.196
Rwork	0.196
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amo
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.610
High resolution limit [Å]	1.880
Number of reflections	113544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	PEG 4000, Sodium Acetate, HEPES