4Y1P
Crystal structure of 3-isopropylmalate dehydrogenase (Saci_0600) from Sulfolobus acidocaldarius complex with 3-isopropylmalate and Mg2+
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-02-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 89.024, 136.719, 112.519 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 33.510 - 2.200 |
| R因子 | 0.19306 |
| Rwork | 0.190 |
| R-free | 0.24961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a05 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.438 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 50.000 |
| 分解能 [Å] (高) | 2.200 |
| 独立反射数 | 35126 |
| <I/σ(I)> | 17.9 |
| 完全性 [%] | 99.7 |
| 冗長性 | 7.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M Ammonium sulfate, 0.1 M Cacodylate (pH6.5), 0.2 M NaCl |
