4WWR
Crystal Structure of Bag6-Ubl4A Dimerization Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.235, 56.494, 75.545 |
| Unit cell angles | 90.00, 96.23, 90.00 |
Refinement procedure
| Resolution | 19.647 - 2.000 |
| R-factor | 0.2267 |
| Rwork | 0.225 |
| R-free | 0.26880 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.650 | 19.650 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.047 | 0.013 | 0.813 |
| Rpim | 0.033 | 0.010 | 0.565 |
| Total number of observations | 78055 | 818 | 5819 |
| Number of reflections | 26499 | ||
| <I/σ(I)> | 13.9 | 49.6 | 1.7 |
| Completeness [%] | 98.6 | 88.7 | 98.2 |
| Redundancy | 2.9 | 2.8 | 3 |
| CC(1/2) | 0.999 | 0.999 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298.13 | 20% PEG-3350, 0.05 HEPES sodium pH 7.0, 1% tryptone |
