4V7E
Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome
This is a non-PDB format compatible entry.
Replaces: 3IZ5Replaces: 3IZ6Replaces: 3IZ7Replaces: 3IZ9Replaces: 3IZREM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 10 seconds before plunging, use 2 layers of filter paper |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.26 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 1374 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.5 |
| Software | SPIDER |
| Symmetry | C1 |
