4UXX
Structure of delta4-DgkA with AMPPCP in 9.9 MAG
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.03320, 1.28238 |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 72.940, 72.940, 195.760 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 53.075 - 2.701 |
| R因子 | 0.2212 |
| Rwork | 0.219 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ze5 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.749 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 53.070 | 2.770 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| Rmerge_l_obs | 0.120 | 0.860 |
| 独立反射数 | 17218 | |
| <I/σ(I)> | 11.9 | 1.6 |
| 完全性 [%] | 99.3 | 94.1 |
| 冗長性 | 9.1 | 5.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 7-10 %(V/V) 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M SODIUM CHLORIDE, 0.1 M LITHIUM NITRATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPID CUBIC PHASE) METHOD AT 4 DEGREES CELCIUS WITH THE 9.9 MONOACYLGLYCEROL (MONOOLEIN, 9.9 MAG) AS THE HOSTING LIPID.CRYSTALS WERE SOAKED WITH 5-10 MM AMPPCP, 60 MM ZINC ACETATE BEFORE HARVESTING. |
