4UA2
Crystal structure of dual function transcriptional regulator MerR from Bacillus megaterium MB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-26 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.0, 0.97914, 0.95370 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.542, 94.806, 87.060 |
Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
Resolution | 28.411 - 2.610 |
R-factor | 0.2267 |
Rwork | 0.225 |
R-free | 0.25740 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.670 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.610 |
Number of reflections | 38508 |
<I/σ(I)> | 24.3 |
Completeness [%] | 99.3 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | sodium citrate, ammonium phosphate |