4RGE
Crystal structure of the in-line aligned env22 twister ribozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 64.071, 64.071, 578.773 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.487 - 2.890 |
R-factor | 0.1796 |
Rwork | 0.177 |
R-free | 0.22290 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.335 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.500 |
High resolution limit [Å] | 2.889 |
Number of reflections | 17191 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 0.1 M imidole, pH 7.9, CaCl2 0.2 M and 14-16% PEG1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |