4R0S
Crystal structure of P. aeruginosa TpbA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 80.138, 82.765, 119.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.990 - 2.030 |
R-factor | 0.22236 |
Rwork | 0.220 |
R-free | 0.25037 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.336 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 25665 | |
Completeness [%] | 99.1 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 293 | 1.7M ammonium phosphate monobasic, 0.1M Tris, 7%(v/v) PEG400, pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |