4Q9C
IgNAR antibody domain C3
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 63 2 2 |
| 格子定数 [Å] | 99.148, 99.148, 52.281 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 15.000 - 2.800 |
| R因子 | 0.25975 |
| Rwork | 0.259 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q97 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.919 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.900 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| Rmerge_l_obs | 0.036 | 0.304 |
| 独立反射数 | 4052 | |
| <I/σ(I)> | 22.5 | 3.6 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.0 M lithium chloride, 0.1 M citric acid, 20% PEG6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
