4Q97
IgNAR antibody domain C1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| 格子定数 [Å] | 41.250, 41.250, 98.340 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 15.000 - 2.400 |
| R因子 | 0.26634 |
| Rwork | 0.265 |
| R-free | 0.28389 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ind |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.500 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| Rmerge_l_obs | 0.056 | 0.393 |
| 独立反射数 | 7190 | |
| <I/σ(I)> | 21.7 | 4.5 |
| 完全性 [%] | 98.3 | 98.2 |
| 冗長性 | 5.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1 M tri-sodium citrate; 0.1 M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
