4Q4Z
Thermus thermophilus RNA polymerase de novo transcription initiation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.487, 102.159, 294.719 |
Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
Resolution | 29.781 - 2.900 |
R-factor | 0.2559 |
Rwork | 0.256 |
R-free | 0.27470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.930 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.850 |
Number of reflections | 111819 |
Completeness [%] | 88.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 300 | crystallization solution [100 mM Tris-HCl, 200 mM KCl, 50 mM MgCl2, 10 mM Spermine tetra HCl and 10% PEG 4000], pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 300K |