4OMF

The F420-reducing [NiFe]-hydrogenase complex from Methanothermobacter marburgensis, the first X-ray structure of a group 3 family member

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-05
Detector	PSI PILATUS 6M
Wavelength(s)	1.0, 1.71
Spacegroup name	F 2 3
格子定数 [Å]	233.160, 233.160, 233.160
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	19.990 - 1.710
R因子	0.152
Rwork	0.150
R-free	0.18100
Structure solution method	cryo-EM model of FrhABG complex and calculated SAD phases were combined
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.317
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	1.700
独立反射数	112689
完全性 [%]	86.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	6.5	281	17-20 % PEE797, 100 mM MES/NaOH, 400 mM MgCl2, 5% glycerol, 100 mM trimethylamine oxide, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 281.0K