4OI3
Crystal structure analysis of SCO4226 from Streptomyces coelicolor A3(2)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 29.574, 66.982, 34.265 |
| 格子定数 [度] | 90.00, 95.02, 90.00 |
精密化法
| 残基 | 34.140 - 1.300 |
| R因子 | 0.15327 |
| Rwork | 0.152 |
| R-free | 0.16926 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.073 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 34.140 | 1.320 |
| 分解能 [Å] (高) | 1.300 | 1.300 |
| Rmerge_l_obs | 0.317 | |
| 独立反射数 | 30687 | |
| 完全性 [%] | 93.9 | 66.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M sodium citrate dehydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
