4OE1
Crystal structure of the pentatricopeptide repeat protein PPR10 (C256S/C430S/C449S) in complex with an 18-nt PSAJ rna element
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 |
| 格子定数 [Å] | 83.318, 83.318, 225.500 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 34.259 - 2.800 |
| R因子 | 0.2636 |
| Rwork | 0.263 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m59 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.999 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 2.900 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| 独立反射数 | 37060 | |
| 完全性 [%] | 98.0 | 99.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 9% PEG 3350, 0.1M MES 5.5, 6% Tacsimate, pH 6.0, 70 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
