4NXH
2'-Trifluoromethylthiouridine-modified E. coli 23S rRNA Sarcin Ricin Loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 |
| Unit cell lengths | 29.560, 29.560, 76.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 1.158 |
| R-factor | 0.117 |
| Rwork | 0.115 |
| R-free | 0.14810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dvz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.740 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.560 | |
| High resolution limit [Å] | 1.158 | 1.158 |
| Number of reflections | 22732 | |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.2 M ammonium sulfate, 50 mM potassium MOPS, pH 7.0, 10 mM magnesium chloride, 10 mM manganese chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
