4NIC
Crystal structure of Klebsiella pneumoniae RstA BeF3-activated N-terminal receiver domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 113.475, 113.475, 243.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.598 - 3.180 |
| R-factor | 0.1906 |
| Rwork | 0.187 |
| R-free | 0.22270 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.116 | 0.354 |
| Number of reflections | 15850 | |
| <I/σ(I)> | 15.689 | 6.8 |
| Completeness [%] | 98.0 | 99.8 |
| Redundancy | 6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 299 | 100 mM Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
