4MYB
Crystal Structure of Francisella tularensis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97870 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 200.409, 200.409, 200.409 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.562 - 2.400 |
R-factor | 0.2551 |
Rwork | 0.255 |
R-free | 0.26560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.267 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.562 | 2.580 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.076 | |
Number of reflections | 13737 | |
<I/σ(I)> | 12.35 | 8.06 |
Completeness [%] | 100.0 | 99 |
Redundancy | 1 | 43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 0.1 M citric acid, pH 4.5, 7% PEG6000, 0.5 M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |