4MSV
Crystal structure of FASL and DcR3 complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| 格子定数 [Å] | 126.909, 126.909, 205.729 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 48.520 - 2.500 |
| R因子 | 0.18914 |
| Rwork | 0.187 |
| R-free | 0.22075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j6g |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.022 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.540 |
| 分解能 [Å] (高) | 2.500 | 2.500 |
| Rmerge_l_obs | 0.116 | 0.826 |
| 独立反射数 | 22326 | |
| <I/σ(I)> | 14.9 | 2 |
| 完全性 [%] | 100.0 | 99.8 |
| 冗長性 | 7.3 | 7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.09M Malonic Acid, 0.013M Ammonium Citr Tribasic, 0.006M Succinic Acid, 0.015M DL-Malic Acid, 0.02M Sodium Acetate, 0.025 M Sodium Formate, 0.008 M Ammonium Tartrate Dibasic, Final pH 7.0; 0.1M HEPES:NaOH pH 7.0, 10% (w/v) PEG MME 5000, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
