4MM0
Crystal Structure Analysis of the Putative Thioether Synthase SgvP Involved in the Tailoring Step of Griseoviridin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 77.15 |
Detector technology | CCD |
Collection date | 2012-05-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979228 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 184.050, 184.050, 80.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.856 - 2.600 |
R-factor | 0.1727 |
Rwork | 0.171 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y46 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.127 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.856 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 42958 | |
Completeness [%] | 99.9 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 277.15 | 20%(w/v) Polyethylene glycol 3350, 0.2M Potassium sodium tartrate tetrahydrate, 3.0%(v/v) Dimethyl sulfoxide, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |